Syllabus Molecular Biology

MOLECULAR BIOLOGY

4(2-2)

THEORY

Introduction to Molecular Biology: Why we need to study molecular biology, Genes and chromosomes, Identification of DNA as the genetic material, Structure of DNA, Replication of DNA, Expression of genetic information, Vectors and their uses in molecular biology: composition of a plasmid vector, M 13 and lamda Phages as vectors in molecular biology, Modifications of phage vectors and their uses, DNA modification enzymes, linkers, adaptors and their uses, Specialized libraries: chromosomal, deletion enrichment, subtractive hybridization libraries, Screening libraries: screening strategies I, Library screening strategies II, Hybridization of nucleic acids, Principle of polymerase chain reaction, Principles of primer designing for PCR, DNA sequencing: principle and methods, Finding of open reading frame in a cloned gene, Introducing mutations in DNA, Cite directed mutagenesis in a given gene, Expression systems and their uses, Promoters and gene expressions, Regulation of gene expression, How to study promoters? How to make fusion proteins, Satellite, minisatellite and micorsattelite DNA, Restriction fragment length polymorphism, How to prepare a transgenic animal?

PRACTICAL

Good lab practices, Genomic DNA extraction from eukaryotic and prokaryotic cells, Plasmid DNA extraction, RNA extraction, Estimation of nucleic acids, Reverse Transcriptase of RNA, PCR, Restriction of DNA, Electrophoresis of DNA in agarose gel, Preparation of cell lysate, Protein estimation, SDS-PAGE, Staining of gel and its documentation, Transformation of host cells, Selection of transformants, Use of computers for DNA and protein sequence data from world wide web.

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BOOKS RECOMMENDED

1. Bell, D.D. and W.D. Weaver. 2002. Commercial Chicken Meat and Egg Production. 5th Edition. Kluwer Academic Publishers, Norwell Massachusetts 02061 USA.

2. Crawford, R.D. 1990. Poultry Breeding and Genetics. Elsevier Science Publishers, Amsterdam. The Netherlands.

3. Etches, R. J. 1996. Reproduction in Poultry. CAB International, UK.

4. Ensminger, M.E. 1992. Poultry Science. The Interstate Printers and Publishers Inc. Danville. Illinois. USA.

5. Muir, W.M. and S.E. Aggrey. 2003. Poultry Genetics. Breeding and Biotechnology. Ist Edition. CAB International, UK.

6. Parkhurst, C.R. and G.J. Mountney. 1997. Poultry Meat and Egg Production. Chapman and hall Inc., New York, USA.

7. Taylor, L.W. 2003. Fertility and Hatchability of Chicken and Turkey Egg. Greenword Publishers. Lucknow. India.

Posted in: 3rd semester